SSEA-4 Antigen Is Expressed on Rabbit Lymphocyte Subsets

SSEA-4 antigen can be mainly found in embryos and embryonic stem cells. However, its expression has been observed also adult progenitor cells, or even some differentiated Moreover, we a considerable number of positive (SSEA-4+) cells within the rabbit peripheral blood bone marrow mononuclear (PBMCs BMMCs) our previous study. Since no information about such anywhere literature, aim this study was to identify their origin. At first, phenotypic analyses fresh PBMCs BMMCs were performed using flow cytometry specific antibodies against leukocyte subsets. Then, SSEA-4+ enriched magnetic activated cell sorting (MACS) analyzed for phenotype qPCR. We significant population (~50%) (~20%). All those co-expressed CD45 majority them expressed B-cell marker (IgM; 50% 60% BMMCs). Increased (p < 0.05) SSEA-4, markers (IgM, CD79α MHCII) noticed by qPCR via MACS (with efficiency over 80%). Both methods did not detect monocyte T-cell markers. In conclusion, are hematopoietic origin probably belong B-lineage as possessing B lymphocytes. true function these should explored further studies.